Journal: Experimental and Therapeutic Medicine

Article Title: Cell sheets of co-cultured BMP-2-modified bone marrow stromal cells and endothelial progenitor cells accelerate bone regeneration in vitro

doi: 10.3892/etm.2019.7982

Figure Lengend Snippet: Characterization of BMSCs and EPCs. (A) Flow cytometry analysis of Ficoll-separated BMSCs surface markers, including CD44, CD71 and CD45. (B) Flow cytometry analysis of Percoll-separated EPCs surface markers, including CD31, CD133 and VEGFR. BMSCs, bone marrow stromal cells; EPCs, endothelial progenitor cells; CD44, CD44 antigen; CD71, transferrin receptor protein 1; CD45, receptor-type tyrosine-protein phosphatase C; CD31, platelet endothelial cell adhesion molecule; CD133, prominin-1; VEGFR, vascular endothelial growth factor receptor; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Article Snippet: Subsequently, primary antibodies purchased from BIOSS, including fluorescein isothiocyanate/phycoerythrin (FITC/PE)-conjugated rat anti-CD44 (cat. no. bs-4916R-FITC), FITC/PE-conjugated rat anti-CD71 (cat. no. bs-1782R-FITC) and FITC/PE-conjugated rat anti-receptor-type tyrosine-protein phosphatase C (CD45) (cat. no. bs-10599R-FITC) at a concentration of 2 mg/ml for the identification of BMSCs; and FITC/PE-conjugated rat anti-CD31 (cat. no. bs-0195R-FITC), FITC/PE-conjugated rat anti-CD133 (cat. no. bs-0395R-FITC) and FITC/PE-conjugated rat anti-VEGFR (cat. no. bs-0170R-FITC) were added to the cells at a concentration of 2 mg/ml and incubated with anti-mouse compensation beads for 30 min in the dark for the identification of EPCs.

Techniques: Flow Cytometry